FastQCFastQC Report
Thu 20 Feb 2020
Mu.S2.3.R2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMu.S2.3.R2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences22179107
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACGG153650.076.195762
GAGTACG184850.064.621881
GTACGGG214550.054.131333
TACGGGG117550.048.450334
TACGGGA107050.036.118564
TACGGGT52150.021.6764534
ACGGGGG79150.020.7402065
TACGGGC65650.019.8511984
ACGGGAC70750.014.8583063
ACGGGGA154400.014.4092155
CCTATAC67050.013.9600653
TATACTG128800.013.6955475
ACGGGGC92550.013.3807725
CTTACAC113500.013.2584673
GTCTAGG57400.013.1713431
TAGGACT81150.012.9540994
TATACAG145500.012.5689755
TACACTG180200.012.5459635
GTATATA124050.012.5374621
TATACCG41250.012.2180875
Produced by FastQC (version 0.11.5)