FastQCFastQC Report
Tue 21 Jan 2020
5X.1.1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename5X.1.1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25412445
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG60700.028.64909494
GGGGGAT66450.018.9556061
GGGGATA85950.014.1082022
CGGGAAC142650.013.7392221
TAGGGCG27650.012.7486438
GTTTTAT165050.012.6434421
TTAATAC60900.011.9622333
CTTTTAT180200.011.6065471
GTTTTTT293050.011.4994031
CTTAAAC172750.011.3452971
CCCCGCG33500.011.2238894
GTAGGGC33300.011.150157
TTAAACG92400.011.139612
TAAACGT90650.010.9917273
CTTTATT160700.010.9383951
TTTAATA105500.010.9146922
TTGAGGT290850.010.6006537
GTTTTTA174750.010.5968521
TTTGAGG324200.010.3365216
GTTTGAT350000.010.3131431
Produced by FastQC (version 0.11.5)