FastQCFastQC Report
Tue 21 Jan 2020
5X.1.2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename5X.1.2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25412445
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG81700.021.40024294
GGGGGAT78050.017.0416411
CGGGAAC118350.015.2893961
GGGGATA102200.012.2788652
GTTTTAT169650.011.9958731
TTAATAC62250.011.4008043
CTTTTAT174700.011.1917571
TAGGGCG29600.011.1148658
CTTAAAC175700.010.9943081
GTTTTTT275800.010.9234951
TTGAGGT280000.010.7932157
GTTTAAT103650.010.7920891
TTTAATA101700.010.6755162
GGGCCCT34350.010.5356638
CGCGTAT19700.010.4974621
ATTTAAT87400.010.3787181
CTTTAAT148200.010.3387321
TTAAACG97100.010.309992
GTTTGAT348400.010.2390931
CGCAAAC105200.09.9629281
Produced by FastQC (version 0.11.5)