FastQCFastQC Report
Tue 21 Jan 2020
5X.2.1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename5X.2.1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25337876
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG66750.031.26292294
GGGGGAT61700.019.957861
CGGGAAC147250.016.1188471
TAGGGCG33400.015.4790428
GGGGATA82250.014.9142882
GTAGGGC38450.013.5682717
CGTGCAT59800.013.046823594
TTGAGGT301950.011.6274217
AGGGCGG46200.011.5974039
GGGAACA196150.011.5014032
GTTTTTT296550.011.4429281
CTTAAAC164800.011.4077671
TTAAACG92100.011.328992
GTTTTAT165150.011.2128371
GGGTAGG45700.011.1072225
TTTGAGG336350.011.053076
TGAGGTT308450.010.7424228
CCCCGCG39100.010.69821194
TTGCGCC56650.010.6195958
ACCGTGC70400.010.6150578
Produced by FastQC (version 0.11.5)