FastQCFastQC Report
Tue 21 Jan 2020
5X.2.2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename5X.2.2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25337876
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTTTTGAGGTTTACACAAAAGCAAAGGGAAATTAACCGGTGAAGCTT261000.10300784485645126No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG84650.024.4855394
GGGGGAT76500.018.1241821
CGGGAAC122050.015.7115941
TAGGGCG33200.013.8734958
GGGGATA107150.012.5450322
GTAGGGC38550.012.0700397
CGCGTAT18850.011.7188331
TTAATAC62400.011.59935953
CGGGGAC74300.011.4495291
TTGAGGT291300.011.1489897
AGGGCGG46800.010.8461549
TTTAATA102650.010.8056512
CGGGAAT98350.010.7046271
CTTTTAT168350.010.6646861
GGGTAGG44700.010.6196875
CTTAAAC178600.010.5789471
CGTGCAT51550.010.5761494
GTTTTAT158600.010.4609091
GTCGGTT69350.010.3013711
ATTTAAT91100.010.266741
Produced by FastQC (version 0.11.5)