FastQCFastQC Report
Tue 21 Jan 2020
5X.3.1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename5X.3.1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25340408
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG67850.033.66543694
GGGGGAT61850.015.4260311
CGGGAAC131950.013.5710511
GTTTTAT173650.011.4488921
CTTAAAC174700.011.299371
GTTTTTT299300.011.2749751
GGGGATA81100.011.1849572
TAAGTCG45600.011.02850994
GTTTAAT100950.010.5685981
TTGAGGT293850.010.4924297
CGTGCAT54100.010.33826394
GTTTGAT350600.010.1748441
TTTGAGG321300.010.1665126
GGAAAAT258000.010.1286821
GTTTATT153650.010.1249591
TTAAACG90850.010.0880572
CTTTTAT182150.09.9857251
CTTTATT160650.09.9763461
TGAGGTT299000.09.76153858
GTTTTTA171200.09.7184571
Produced by FastQC (version 0.11.5)