FastQCFastQC Report
Tue 21 Jan 2020
5X.3.2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename5X.3.2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25340408
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG89550.025.87493194
CGGGAAC109600.015.1806561
TAGGGCG30250.013.6727278
GGGGGAT73050.012.1601651
GTAGGGC34500.012.1246377
TTTAATA103250.011.7898312
ATTTAAT89000.011.6179771
TTAATAC63950.011.6121973
CTTAAAC176000.010.9221591
GTTTTTT274200.010.8158281
GTTTTAT168400.010.4940611
GTTTAAT103850.010.4092441
CTTTTAT173000.010.3508671
AGGGCGG42750.010.3345039
TTGAGGT285600.09.972697
CTTTATT149550.09.9311271
CGAAAAT134250.09.8726251
CGGGAAT94550.09.7927021
GGGTAGG42400.09.7547175
CTTAAAT101500.09.6778341
Produced by FastQC (version 0.11.5)