FastQCFastQC Report
Tue 21 Jan 2020
Ler.1.1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLer.1.1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25329602
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTTTTGAGGTTTACACAAAAGCAAAGGGAAATTAACCGGTGAAGCTT253300.10000157128406519No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG65900.030.23975694
GGGGGAT68050.016.506981
CGGGAAC131800.013.5151741
CCCCGCG36250.012.70620794
CTTAAAC162200.012.2281131
GGGGATA90100.011.997782
GTTTTAT164000.011.9792671
GTTTTTT295350.011.966821
CGTGCAT57800.011.87197294
TTTAATA103750.011.7330122
TTAATAC63350.011.7221783
TTAAACG88950.011.5188312
TTGAGGT293800.011.4220557
GTTTTTA165450.010.9652461
TTTGAGG332450.010.8999846
CTTTAAT144600.010.7911471
CTTAGAT116150.010.6018081
CTTTTAT175200.010.5964621
AGTTTTG308400.010.5612843
CGCGTAT21000.010.5190491
Produced by FastQC (version 0.11.5)