FastQCFastQC Report
Tue 21 Jan 2020
Ler.1.2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLer.1.2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25329602
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTTTTGAGGTTTACACAAAAGCAAAGGGAAATTAACCGGTGAAGCTT268300.10592349615284125No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG93950.021.16125594
CGGGAAC111850.013.7407241
TTAATAC68000.013.6852943
TTTAATA107400.013.3473012
CAGACGT54950.013.2575073
GGGGGAT78300.013.205621
TAGGGCG31200.012.0512828
TTGAGGT292500.011.8102577
ATTTAAT98950.011.7796861
CGTGTGC63850.011.7776037
GACGTGT59700.011.7303185
CGCGTAT19400.011.3865981
GTTTTTT274000.011.1496351
GTTTTAT169950.011.06207751
CTTAAAC171450.010.9378821
TTTGAGG348200.010.7174036
GTTTTTA159250.010.683831
GTAGGGC36250.010.6317247
TAATACA96150.010.5585024
GTTTGAT344600.010.44747451
Produced by FastQC (version 0.11.5)