FastQCFastQC Report
Tue 21 Jan 2020
Ler.2.1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLer.2.1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25466584
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG64050.024.72911894
GGGGGAT69950.013.3709781
CGCGTAT20300.012.9655151
CTTAAAC166050.012.7654311
GTTTTAT169550.012.723681
CGGGAAC137650.012.5993451
GTTTTTT295350.011.8713391
TTAAACG86750.011.2691642
CCCCGCG34500.011.17101494
CTTTTAT180450.010.9393181
GTTTGAT340850.010.7278861
GTTTTTA163000.010.6687111
TAGGGCG30600.010.5980398
GGGGATA88400.010.5271482
CTTTATT159100.010.5166551
GGAAAAT249750.010.4068071
TTTGAGG319800.010.4052526
CTCGAAT176000.010.3880681
CTTAGAT113800.010.3251311
GTCGGTT69700.010.1822091
Produced by FastQC (version 0.11.5)