FastQCFastQC Report
Tue 21 Jan 2020
Ler.2.2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLer.2.2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25466584
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG90900.017.68316894
CGGGAAC115100.013.8427451
TTAATAC64250.013.2404663
TTTAATA105700.012.5837272
ATTTAAT96550.012.5592961
GGGGGAT81800.012.3533011
GTTTTAT164950.011.3404061
CTTAAAC178050.011.2715521
TTGAGGT296600.011.0131497
TTAAACG92900.010.7255112
GTTTTTT279100.010.7101391
CTTTTAT172650.010.6985231
CGCAAAC102150.010.4904551
TTTGAGG349650.010.484776
CGCGAAC36050.010.4299571
GTTTTTA167350.010.363311
TAATACA93400.010.06423954
CTTTATT147900.010.0101411
GTTTTAC191200.09.9801251
TGAGGTT319250.09.8931878
Produced by FastQC (version 0.11.5)