FastQCFastQC Report
Tue 21 Jan 2020
Ler.3.1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLer.3.1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25488885
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG65150.022.58020294
CGCGTAT20350.014.7813261
CCCCGCG40500.012.88148294
CGGGAAC142600.012.6563821
CTTAAAC170650.012.5314981
GTTTTTT294400.012.4843761
GGGGGAT72400.012.4640881
GTTTTAT155800.012.3080871
TAAACGT91500.012.1224053
TTAAACG94100.011.9872482
TTTAATA101850.011.6750132
TAGGGCG33000.011.393948
CTTTAAT144050.011.12599851
GTAGGGC36900.010.6991887
CTTTTAT177350.010.5210051
CTCGAAT179050.010.4473621
GTTTGAT337700.010.3547531
GTTTTTA164750.010.3271641
CGTTTTA89700.010.32218557
AGGGCGG40750.010.2650329
Produced by FastQC (version 0.11.5)