FastQCFastQC Report
Tue 21 Jan 2020
Ler.3.2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLer.3.2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25488885
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTTTTGAGGTTTACACAAAAGCAAAGGGAAATTAACCGGTGAAGCTT270450.10610507285822819No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG92150.015.86218294
CGGGAAC118150.015.3550571
CGCGTAT20450.013.7897311
GGGGGAT84750.012.588791
TAGGGCG35500.012.5774658
CTTAAAC173250.012.072151
TTAATAC60250.012.0132793
GTTTTTT261350.011.6173721
TTTAATA101150.011.6164122
AGGGCGG42200.011.5829389
TTAAACG92400.011.5465382
GTTTTTA152850.011.4079171
TTGAGGT305950.011.2296137
CCCCGCG51100.011.12915994
GTAGGGC39500.011.0658247
TAAACGT90000.010.7577783
AGTTTTG302250.010.6517783
GTTTTAT166000.010.5325311
TTTGAGG361250.010.4993776
GTCGGTT72800.010.3942311
Produced by FastQC (version 0.11.5)