FastQCFastQC Report
Tue 21 Jan 2020
Mp.1.1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMp.1.1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25824818
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG59700.031.33333494
GGGGGAT65400.015.9541281
CGGGAAC137850.013.1606821
CCCCGCG42000.012.75714294
GTTTTAT168350.012.0885061
TAGGGCG31400.011.9745238
GGGGATA85700.011.9556592
GTTTTTT311100.011.4214081
GTTTTTA176800.011.2183271
TTAAACG87150.011.0556522
TTGCGCC54100.010.7726438
TTGAGGT312350.010.7136227
CTTAAAC166500.010.670271
GTTTAAT107250.010.5613051
CGTGCAT54750.010.55890494
CTCGAAT166850.010.5070421
GTAGGGC37900.010.4168877
TTTGAGG345600.010.3764476
CTTTAAT151900.010.3653721
TAAACGT88450.010.3617863
Produced by FastQC (version 0.11.5)