FastQCFastQC Report
Tue 21 Jan 2020
Mp.1.2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMp.1.2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25824818
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTTTTGAGGTTTACACAAAAGCAAAGGGAAATTAACCGGTGAAGCTT283070.10961161468785569No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG83250.022.35675694
CGGGAAC116200.014.4397591
TAGGGCG33250.013.4285728
TTAAACG98050.013.182052
TTAATAC62000.012.9629033
CTTAAAC194300.012.6994331
TAAACGT93400.012.5299783
TTGAGGT309800.012.4554557
GGGGGAT84950.012.3378461
CGCGTAT17150.012.3323621
TTTAATA102050.011.882412
AGTTTTG313950.011.721933
CTTTTAT176650.011.4939151
ATTTAAT88600.011.4582391
TTTGAGG360150.011.3405536
GTTTTTT266400.011.3089341
GTAGGGC38900.011.2365047
TGAGGTT329600.011.1938728
TTTTGAG395150.010.9545745
GAGGTTT330900.010.8942299
Produced by FastQC (version 0.11.5)