FastQCFastQC Report
Tue 21 Jan 2020
Mp.2.1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMp.2.1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25417652
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG62950.031.20889794
GGGGGAT68800.016.1904091
CGGGAAC139350.014.6716911
GTTTTAT169750.012.3210611
TAGGGCG31000.011.977428
GTTTTTT291500.011.931391
CCCCGCG40600.011.80788194
GGGGATA84850.011.7984692
CTTAAAC171150.011.4513581
CGTGCAT54850.010.96809594
GTTTAAT98250.010.7633591
TTAAACG88700.010.7034962
TTTAATA97800.010.57259752
CTTTAAT155450.010.3403021
TTGAGGT289250.010.3180657
GTTTGAT347500.010.2656131
CTCGAAT164400.010.1490271
CTTTTAT180750.010.141081
GTAGGGC35750.010.1230777
GGGAACA182400.09.99780752
Produced by FastQC (version 0.11.5)