FastQCFastQC Report
Tue 21 Jan 2020
Mp.2.2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMp.2.2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25417652
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG82150.023.97200294
CGGGAAC117500.014.2000011
TTAATAC58150.013.3362013
GGGGGAT76650.013.3059371
TAGGGCG30700.012.70684058
TTTAATA100400.012.264942
CTTAAAC178800.011.7762861
ATTTAAT87850.011.7165621
TTGAGGT288150.011.6133967
CTTTTAT165100.011.0454271
GTTTTTT268400.010.997021
TTAAACG93800.010.7729222
TTTGAGG341200.010.7306576
AGTTTTG305550.010.3829163
GTTTTAT161700.010.3766231
TAAACGT89850.010.3049543
CGCGTAT16900.010.2899411
GTTTAAT96950.010.2774631
GTTTTTA162950.010.2104941
TTTTGAG376000.010.25
Produced by FastQC (version 0.11.5)