FastQCFastQC Report
Tue 21 Jan 2020
Mp.3.1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMp.3.1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25456129
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG60650.027.12283594
GGGGGAT64600.018.3343661
CGGGAAC136500.014.013921
GGGGATA86550.013.5216652
GTTTTTT298050.012.5364871
CGTGCAT56650.012.1959494
TTTAATA101350.011.5471142
GTTTTAT173950.011.4831851
CTTAAAC172700.011.3213661
TTGAGGT288750.011.1986157
TAGGGCG29000.011.1827588
CGCGTAT19500.011.087181
TTAAACG92200.011.0618222
TTAATAC62150.010.9654053
TTTGAGG326350.010.9453056
GTTTGAT342700.010.7522611
ACCGTGC68850.010.7175038
CGCGAAC33600.010.6309521
TGAGGTT299450.010.4688598
CCCCGCG35050.010.3252594
Produced by FastQC (version 0.11.5)