FastQCFastQC Report
Tue 21 Jan 2020
Mp.3.2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMp.3.2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25456129
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTTTTGAGGTTTACACAAAAGCAAAGGGAAATTAACCGGTGAAGCTT264080.10373926059221338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG80550.020.94723794
GGGGGAT80100.016.664171
CGGGAAC118250.016.0575051
GGGGATA103800.012.1348752
GGGCCCT34850.011.8680068
CGCGTAT17600.011.4829551
GTTTTAT165500.011.4163131
TTGAGGT300700.011.2224817
TAGGGCG30750.011.1577238
GTTTAAT95050.010.8290381
CTTAAAC177600.010.5591211
GTTTTTT265950.010.4974621
TTTAATA105050.010.4693012
GTTTGAT349000.010.4638971
TTAATAC62950.010.4527413
GGGAACA166650.010.4350432
CGTGCAT49450.010.264914594
TGTAGGC184700.010.2041153
GCGCCCG77300.010.15394668-69
GTTTTTA162950.010.1528081
Produced by FastQC (version 0.11.5)