FastQCFastQC Report
Tue 21 Jan 2020
PRO.1.1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePRO.1.1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25758935
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG65850.027.55049394
GGGGGAT67700.017.7725261
CGCGTAT21250.014.8188241
TAGGGCG36300.013.5950428
CGGGAAC142950.013.4473591
CGTGCAT55250.013.35565694
GTAGGGC37700.013.2148547
GGGGATA90850.012.3643362
GTTTTAT168500.011.8267051
GTTTTTT299600.011.6401861
CTTAAAC172400.011.4228531
TTAAACG90600.011.3609272
AGGGCGG46150.011.1007599
CTTTATT157600.011.0640871
TTGAGGT291000.010.9182137
CTTTTAT185050.010.8705751
GTTTGAT353900.010.8369591
CCCCGCG37000.010.79729794
CTTTAAT145400.010.7964241
GGGTAGG46300.010.760265
Produced by FastQC (version 0.11.5)