FastQCFastQC Report
Tue 21 Jan 2020
PRO.1.2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePRO.1.2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25758935
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTTTTGAGGTTTACACAAAAGCAAAGGGAAATTAACCGGTGAAGCTT273010.10598652467580666No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG90150.020.48918394
GGGGGAT80600.015.627791
TAGGGCG34400.015.5755828
CGCGTAT19850.014.68011
GTAGGGC37450.014.5580767
CGGGAAC124850.013.3263921
TTAATAC62050.012.6494753
TTGAGGT291550.012.6064147
AGGGCGG45600.012.3684229
AGTTTTG292450.011.7640623
GTTTTTT275600.011.751
TTTAATA107500.011.4548852
GTTTTAT168800.011.30450251
TGAGGTT311800.011.1998088
GGGCCCT34550.011.1548488
GGGTAGG46850.011.1355395
TTAAACG94650.011.0734282
CTTTATT153950.011.05164051
GAGGTTT316050.010.9748469
CTTAAAC174350.010.9176941
Produced by FastQC (version 0.11.5)