FastQCFastQC Report
Tue 21 Jan 2020
PRO.2.1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePRO.2.1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25730074
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTTTTGAGGTTTACACAAAAGCAAAGGGAAATTAACCGGTGAAGCTT308980.12008515793619559No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG74700.027.87282694
GGGGGAT75600.018.7129651
CGCGTAT22150.016.1264111
GTAGGGC40250.014.4795047
TAGGGCG42150.014.2728358
CGGGAAC153950.013.3108161
AGGGCGG49500.012.9131329
AGTTTTG301100.012.8153443
TTGAGGT316150.012.7107397
GGGGATA109700.012.6390162
TTTGAGG355600.012.622336
GGGTAGG49200.012.4186995
TTAAACG83050.012.2805552
CGCGCGT23300.012.1030041
CTTTTAT192400.012.0919961
CTTAAAC158350.012.0802031
GAGGTTT325900.012.02761659
CGTGCAT53500.011.94766494
GGGCAGG73400.011.9100825
TGAGGTT325850.011.6832918
Produced by FastQC (version 0.11.5)