FastQCFastQC Report
Tue 21 Jan 2020
PRO.2.2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePRO.2.2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25730074
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTTTTGAGGTTTACACAAAAGCAAAGGGAAATTAACCGGTGAAGCTT279640.10868215925068851No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG110750.018.8424494
CGCGTAT23000.018.81
CGGGAAC128950.016.2923621
GGGGGAT81450.015.7532231
GCGTGCG33900.012.4778772
GGGCAGG63700.012.2480385
GTTTTTT265100.011.6303291
CGCGCGT20150.011.4292811
GTAGGGC37850.011.2998697
TTGAGGT291200.011.2819387
GCACGTA45650.011.2223448
TAGGGCG39100.011.0588248
CTTAAAC171750.011.028241
GTTTTAT161600.010.906561
GGGCCCT35000.010.8771446
CTTTTAT184250.010.815741
TTTAATA99950.010.8154082
TTAAACG97150.010.7884722
AGTTTTG285200.010.7282613
TAAACGT92750.010.5401623
Produced by FastQC (version 0.11.5)