FastQCFastQC Report
Tue 21 Jan 2020
PRO.3.1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePRO.3.1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25786077
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG70650.028.20665494
CGCGTAT22700.014.9074891
GGGGGAT70700.013.9603971
GTTTTAT161750.012.2040191
CGGGAAC149200.011.9075081
GTTTTTT296500.011.9045531
GTTTTTA164600.011.7642771
CTTAAAC165000.011.4509091
TTAAACG89250.011.2168072
TAAACGT86850.011.0397233
GGGCCCT42050.010.8418568
CTTAGAT114350.010.6864891
CTTTTAT188250.010.6358581
CTTTAAT143950.010.5786731
CGTGCAT53100.010.53295694
CTTTATT156950.010.4810461
TTTGAGG326250.010.3003836
TTGAGGT284100.010.2734967
GTTTGAT351650.010.2380211
CTCGAAT159050.010.1949061
Produced by FastQC (version 0.11.5)