FastQCFastQC Report
Tue 21 Jan 2020
PRO.3.2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePRO.3.2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25786077
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG98750.020.2278594
CGCGTAT20450.017.2371641
CGGGAAC124400.014.0546631
CTTAAAC171700.011.4694231
GTTTTTT271900.011.4431781
CTTTTAT180950.010.9350651
TTTAATA101050.010.837212
GGGGGAT79050.010.7615431
TTAAACG92550.010.715292
GTTTAAT99150.010.6656581
TTGAGGT282000.010.6166677
CTTTAAT137450.010.2582761
TAAACGT91150.010.1579823
CAAGTCG100000.010.15294
GTTTTTA162550.010.0043071
GTAGGGC33850.09.9970467
CGGAAAT139650.09.8947371
GTTTTAT169250.09.8581991
TTAATAC61100.09.8461543
CGGGAAT106800.09.813671
Produced by FastQC (version 0.11.5)