FastQCFastQC Report
Tue 21 Jan 2020
Pa.1.1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePa.1.1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25851691
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTTTTGAGGTTTACACAAAAGCAAAGGGAAATTAACCGGTGAAGCTT295750.11440257428421219No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG66100.030.71709894
GGGGGAT65200.017.5889571
GGGGATA84500.013.2378712
TAGGGCG28300.013.1201428
TTAATAC66850.013.0067333
CCCCGCG36150.012.221394
CGGGAAC134350.012.2091551
CTTAAAC174200.012.0872561
TTGAGGT308700.012.058317
TTTGAGG345000.011.9202916
GTTTTAT172200.011.7090591
GTTTTTT311550.011.6010281
TTTAATA112600.011.5204262
AGTTTTG323200.011.19743
TGAGGTT327600.010.9896228
GAGGTTT337050.010.890679
CGTGCAT53150.010.78833694
CTTAGAT113750.010.7841761
ATTTAAT98600.010.7251521
GTAGGGC34350.010.6724897
Produced by FastQC (version 0.11.5)