FastQCFastQC Report
Tue 21 Jan 2020
Pa.1.2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePa.1.2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25851691
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTTTTGAGGTTTACACAAAAGCAAAGGGAAATTAACCGGTGAAGCTT323100.12498215300500073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG85450.023.37624494
TTAATAC68200.015.8504413
CGGGAAC111200.014.4127711
ATTTAAT98450.014.0355531
TTTAATA106300.013.9275652
GGGGGAT81050.013.8593461
TTGAGGT304250.012.1265417
CTTAAAC176450.011.7733081
TTAAACG87050.011.3383112
CGCGAAC31950.011.3270741
GTTTTAT173500.011.1878981
CTTTTAT172500.011.1710151
TTTGAGG362750.011.1556186
TAATACA101000.011.1217824
AGTTTTG320500.011.0864293
CGCGTAT19250.010.9870131
GTTTAAT102200.010.7612521
TGAGGTT334950.010.7204068
CTTTAAT152300.010.677611
CTTTATT158750.010.5990561
Produced by FastQC (version 0.11.5)