FastQCFastQC Report
Tue 21 Jan 2020
Pa.2.1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePa.2.1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25763291
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTTTTGAGGTTTACACAAAAGCAAAGGGAAATTAACCGGTGAAGCTT275000.10674102155660159No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG67400.028.86943894
GGGGGAT73000.015.7095891
CGGGAAC141750.012.898061
GGGGATA91450.012.7457642
TTTAATA103350.012.5970012
TTAATAC62400.012.2772453
GTTTTTT295250.012.1300591
TTAAACG94000.011.852
CTTAAAC173200.011.8314091
CCCCGCG36350.011.24896994
TTTGAGG339350.011.0384566
TTGAGGT307200.011.0309267
GTTTTAT169700.010.9398941
CTTTTAT182150.010.9146311
TAAACGT95100.010.7739233
CGGGAAT101200.010.7282611
CGTGCAT56700.010.69312294
GTTTTTA165800.010.6586251
AGTTTTG318950.010.5066633
GTTCATT157350.010.3050531
Produced by FastQC (version 0.11.5)