FastQCFastQC Report
Tue 21 Jan 2020
Pa.2.2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePa.2.2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25763291
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTTTTGAGGTTTACACAAAAGCAAAGGGAAATTAACCGGTGAAGCTT283190.10991996325314185No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG87700.022.29418894
TAGGGCG32150.014.6189758
CGGGAAC119100.014.4433261
TTAATAC65250.013.7578543
GGGGGAT83200.013.3317311
GTAGGGC37350.012.3319957
AGGGCGG45450.011.788789
TTGAGGT300500.011.746097
TTTAATA105900.011.7167152
ATTTAAT96300.011.7133961
CGGAAAT145800.011.7016461
CGCGTAT19550.011.29923251
CTTAAAC182850.011.2584091
GTTTTTT253000.011.1462461
GTTTTAT165300.010.86146451
TAAACGT92650.010.855913
TTAAACG98400.010.7947162
TGAGGTT323050.010.591558
GGGTAGG44200.010.527155
TTTGAGG357250.010.5248446
Produced by FastQC (version 0.11.5)