FastQCFastQC Report
Tue 21 Jan 2020
Pa.3.1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePa.3.1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25764024
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTTTTGAGGTTTACACAAAAGCAAAGGGAAATTAACCGGTGAAGCTT271070.10521260188237677No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG62100.028.30595894
GGGGGAT70100.014.6162621
CGGGAAC140850.014.0816481
TTAATAC59550.012.1544923
CCCCGCG39100.012.14066594
GTTTTTT289100.011.7703221
TTTGAGG329950.011.5808466
GTTTTAT166050.011.4917191
CTTAAAC169300.011.46544551
TAGGGCG33300.011.291298
TTGAGGT303550.011.2874327
CTTTTAT183000.011.1464471
CTCGAAT165400.010.9117291
GTTTGAT354600.010.8818381
GTTTTTA169650.010.7768941
GTTTAAT101350.010.7587571
GGGGATA87050.010.63641552
CTTTAAT147450.010.6144461
AGTTTTG308200.010.5071383
TTTAATA99150.010.3812412
Produced by FastQC (version 0.11.5)