FastQCFastQC Report
Tue 21 Jan 2020
Pa.3.2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePa.3.2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25764024
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTTTTGAGGTTTACACAAAAGCAAAGGGAAATTAACCGGTGAAGCTT282510.10965290204666786No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG80800.021.28960294
CGGGAAC116450.014.7720051
TTAATAC62500.013.61123
TTTAATA99600.012.6465852
TAGGGCG30800.012.5129878
ATTTAAT89000.012.4629211
GGGGGAT86200.012.0498841
GTAGGGC34400.012.0232557
TTGAGGT297650.012.0006717
CTTAAAC185050.011.9627121
TAAACGT93600.011.8002143
TTAAACG95600.011.5533482
CTTTTAT166000.011.1837351
TTTGAGG355850.010.8700296
GTTTTTT257400.010.8278941
AGTTTTG311150.010.8153633
CTTTAAT148250.010.683981
TGAGGTT327600.010.4444448
CTTTATT147500.010.41966151
TTTTGAG386400.010.35119155
Produced by FastQC (version 0.11.5)