FastQCFastQC Report
Tue 21 Jan 2020
RGA.1.1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRGA.1.1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25809783
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG63150.034.0126794
CGGGAAC141050.014.2282881
GGGGGAT67100.013.7988071
CTTAAAC175950.011.6732021
TTAAACG91500.011.6601092
GTTTTTT293850.011.5640631
TAGGGCG29850.011.4941378
GTTTTAT171450.011.4587341
TTAATAC63850.010.8206733
CGTGCAT54800.010.80656994
TAAACGT91900.010.7399343
CTTTTAT185500.010.717521
CTTTATT157950.010.6527381
TTTAATA104850.010.489272
GTTTTTA172050.010.4626561
GGGGATA85900.010.1769492
CGCGTAT22300.010.1165911
TTTGAGG319550.010.0751056
CCCCGCG33250.010.0360994
GGAAAAT256300.09.9758091
Produced by FastQC (version 0.11.5)