FastQCFastQC Report
Tue 21 Jan 2020
RGA.1.2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRGA.1.2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25809783
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG85100.025.6263294
CGGGAAC120900.014.0727861
TAGGGCG30750.013.2975618
GGGGGAT77650.012.9529941
TTAATAC64050.012.1811083
ATTTAAT96000.011.7989581
TTTAATA103100.011.715812
CTTAAAC182950.011.2779451
TTAAACG98100.011.1151892
GTAGGGC36550.011.0588237
GGGCCCT34500.011.0347828
GTTTTTT268850.010.7863111
TAAACGT94350.010.6104933
TTGAGGT288100.010.4408197
AGGGCGG43800.010.4086769
CGCAAAC98450.010.4073121
CTTTTAT173250.010.3901861
TAAGTCG48550.010.26158594
GTTTTAT182850.010.2559461
CTTTAAT147250.010.245841
Produced by FastQC (version 0.11.5)