FastQCFastQC Report
Tue 21 Jan 2020
RGA.2.1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRGA.2.1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25784901
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG63450.028.51851894
CCCCGCG42150.013.15776994
CGGGAAC144600.013.0013841
CTTAAAC166550.012.3884731
GTTTTTT296550.012.3463151
TTAAACG82550.012.2982
TAGGGCG29500.011.9491538
GGGGGAT64150.011.8690571
GTTTTTA169250.011.2466761
CTTTAAT147300.011.2315011
TTGAGGT290500.011.1311537
GTTTTAT166700.011.0803831
TTTAATA102450.011.0102492
GTTTTAC193200.010.7769161
TAAACGT87300.010.7674683
CTTAGAT109000.010.6935781
CTCGAAT170450.010.5884431
GGAAAAT250400.010.5487221
CTTTTAT177150.010.5328821
TTAATAC60500.010.4876023
Produced by FastQC (version 0.11.5)