FastQCFastQC Report
Tue 21 Jan 2020
RGA.2.2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRGA.2.2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25784901
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTTTTGAGGTTTACACAAAAGCAAAGGGAAATTAACCGGTGAAGCTT259280.1005549720745486No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG85750.021.15684994
TTAATAC65150.015.3660783
CGGGAAC119300.015.3252311
TAGGGCG34200.013.8801168
TTTAATA105300.013.3456792
ATTTAAT94150.012.3802441
GTTTTTT271850.012.20599651
CTTAAAC184400.012.10683251
GTAGGGC40050.011.6179787
CCCCGCG57050.011.61612794
TTAAACG95050.011.5707522
TTGAGGT299250.011.4182137
TAAACGT91400.011.2614883
CTTTTAT166250.010.9972931
CTTTAAT148400.010.9898911
TAATACA95650.010.9085224
GTTTTAT167300.010.8720861
GTTTTAC196600.010.6383521
AGGGCGG47350.010.6209089
GGAAAAT261450.010.5523041
Produced by FastQC (version 0.11.5)