FastQCFastQC Report
Tue 21 Jan 2020
RGA.3.1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRGA.3.1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25757458
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTTTTGAGGTTTACACAAAAGCAAAGGGAAATTAACCGGTGAAGCTT267440.10383012174570952No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG64650.031.18793794
GGGGGAT73350.017.4287661
TAGGGCG30300.014.5808588
CGGGAAC146900.013.2137521
CGTGCAT53750.012.41674594
GTAGGGC37650.012.3585667
GGGGATA95350.012.2737282
GTTTTTT292550.011.727911
TTAATAC62900.011.5071553
AGGGCGG43200.011.4236129
GTTTTTA169300.011.3821621
CCCCGCG36050.011.34258194
TTTAATA103450.011.2218472
GTTTTAT163350.011.1349871
TTGAGGT306200.011.0822997
CTTAAAC176800.010.9790731
GTTTGAT350350.010.9333531
ACCGTGC76400.010.6426728
GTTTAAT102300.010.6129041
TTTGAGG336700.010.5948926
Produced by FastQC (version 0.11.5)