FastQCFastQC Report
Tue 21 Jan 2020
RGA.3.2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRGA.3.2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25757458
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTTTTGAGGTTTACACAAAAGCAAAGGGAAATTAACCGGTGAAGCTT276320.10727766691883958No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG83400.023.83813394
CGGGAAC122300.015.7563371
TTAATAC66950.014.8827493
GGGGGAT83950.014.2763551
TTTAATA108700.012.4526222
ATTTAAT97950.012.4277711
GGGCCCT35500.012.3126768
TAGGGCG31350.011.9936218
GTTTTTT265250.011.8363811
GTTTTAT168500.011.6035611
CTTTTAT168750.011.281
TTGAGGT296550.011.2685897
TAAACGT92500.010.8735133
CTTAAAC191000.010.8026181
TTAAACG102050.010.6849592
CGTGCAT52150.010.63470894
GGGGATA108050.010.57010652
TAATACA97050.010.5090164
AGTTTTG306350.010.3558033
CGCGTAT20550.010.2919711
Produced by FastQC (version 0.11.5)