FastQCFastQC Report
Tue 21 Jan 2020
Sm.1.1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSm.1.1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25760652
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG59600.030.99161194
GGGGGAT68850.018.1583161
TAGGGCG33650.014.6656768
CGGGAAC144700.014.2266761
GGGGATA85550.013.5149042
GTAGGGC38750.012.4929047
GTTAAAT96200.011.6767161
GTTTTTT291400.011.5967741
CGTGCAT55650.011.486073594
GTTTTAT172950.011.2778261
AGGGCGG47800.011.2092069
TTGAGGT293550.011.01557
GTTTTTA172900.010.8461531
CTTTTAT182350.010.8253361
TTTGAGG328000.010.6609766
TTTAATA104200.010.6449142
GGGTAGG46850.010.5336185
GTTTGAT353900.010.3854211
CTTAAAC174150.010.363481
AGTTTTG309100.010.0659993
Produced by FastQC (version 0.11.5)