FastQCFastQC Report
Tue 21 Jan 2020
Sm.1.2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSm.1.2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25760652
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG80200.023.26558794
GGGGGAT80950.016.3150121
CGGGAAC118150.016.1904351
TTAATAC61000.012.4049193
TAGGGCG30650.012.1141938
GGGGATA105950.011.9773482
CTTAAAC186850.011.4953171
TTTAATA99600.011.3253022
GTTTTAT173600.010.9377881
GTTTTTT262300.010.7689681
TTAAACG105900.010.6515592
CTTTAAT149350.010.3535321
TAAACGT96400.010.33613
TTGAGGT290200.010.23577
CGGAAAT146050.010.2013021
CTTTTAT168200.09.9476821
ATTTAAT95950.09.9437211
CGCGTAT19600.09.8316331
GTTTATT147750.09.8294421
TTTGAGG335000.09.7647766
Produced by FastQC (version 0.11.5)