FastQCFastQC Report
Tue 21 Jan 2020
Sm.2.1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSm.2.1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25839527
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG64800.030.89814894
GGGGGAT68150.016.9655171
TAGGGCG34900.014.2750728
CGGGAAC140650.014.0682541
GGGGATA88200.012.5226762
CGTGCAT53200.011.7594
GTTTTAT167900.011.7290061
GTTTTTT300050.011.5914021
GTAGGGC40200.011.3407967
CTTTATT156500.011.0217261
TTGAGGT299300.010.9609097
CTTAAAC176250.010.881
TTAAACG92300.010.6933912
TTTGAGG334600.010.4647346
CTTTTAT187600.010.3971211
CGAAAAT138250.010.2329111
GGGTAGG45950.010.2285095
GGAAAAT254600.010.1716421
GTTTTTA176050.010.1715421
TTGCGCC56150.010.1282288
Produced by FastQC (version 0.11.5)