FastQCFastQC Report
Tue 21 Jan 2020
Sm.2.2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSm.2.2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25839527
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG83650.024.21637794
GGGGGAT80350.014.5650291
CGGGAAC119400.014.5644891
TAGGGCG34350.012.8617178
TTAATAC66800.012.8053893
CGCGTAT18450.011.9728991
GTAGGGC38900.011.5989727
TTTAATA108250.011.4189392
ATTTAAT90450.011.1719191
GTTTTTT271850.010.8920361
GGGGATA106850.010.5128682
CTTTTAT175800.010.3998861
CTTAAAC187150.010.3718941
CGTGCAT51750.010.26280294
TAAACGT98450.010.2163533
GTTTAAT102950.010.1350171
GTTTTTA171000.010.0871351
CTTTATT147450.010.0406921
TTAAACG102400.010.0058592
AGGGCGG47600.09.9726899
Produced by FastQC (version 0.11.5)