FastQCFastQC Report
Tue 21 Jan 2020
Sm.3.1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSm.3.1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25959884
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG67600.032.12130494
CGGGAAC143750.015.3996531
GGGGGAT66800.015.1976051
TAGGGCG37250.013.5006728
GTAGGGC41350.012.38935857
CCCCGCG40450.012.20024794
GTTTTTT302150.012.0086061
CTTTATT159550.011.783141
GGGGATA84650.011.6597762
CGCGAAC33200.011.3253021
GTTTTTA173400.011.2756631
CTTAAAC159450.011.230481
CGTGCAT57250.011.16506794
GTTTTAT171850.010.9397741
GTTTAAT108200.010.6857681
GGGTAGG49150.010.6144475
TTTGAGG337600.010.5248826
AGGGCGG51200.010.4648449
GGGAACA195850.010.3911172
TAAGTCG48800.010.20901794
Produced by FastQC (version 0.11.5)