FastQCFastQC Report
Tue 21 Jan 2020
Sm.3.2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSm.3.2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25959884
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG86950.025.29729794
CGGGAAC118750.015.7920011
TTAAACG102200.013.1986312
GGGGGAT72200.013.0844891
CTTAAAC187250.012.4998671
TAGGGCG34250.012.2131398
TAAACGT100050.011.8850573
TTTAATA102850.011.8813822
TTAATAC62850.011.591093
GTTTTTT269650.011.4689421
TAAGTCG50200.011.42231294
GTTTAAT104350.011.3953051
GTAGGGC37850.010.9273457
CGTTTTA102950.010.591557
ATTTAAT90350.010.4039851
CTTTAAT143850.010.3246441
GGAAAAT261650.010.310721
GTTTTAT179350.010.2202411
TTGAGGT281700.010.0273357
CGTGCAT52200.09.81417794
Produced by FastQC (version 0.11.5)