FastQCFastQC Report
Tue 21 Jan 2020
5X.1.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename5X.1.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24417254
Sequences flagged as poor quality0
Sequence length20-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG53750.030.22713994
GGGGGAT65650.016.8800931
GGGGATA83100.013.167382
TAGGGCG28050.013.1137478
CGGGAAC133050.012.9834951
CGTGCAT52000.012.98182894
GTTTTAT159000.011.4794611
GTAGGGC31700.011.4569217
CTTAAAC163000.010.9692321
CTTTATT156400.010.8367051
GTTTTTT273150.010.7562471
TTTAATA99200.010.5609622
TTAATAC62050.010.4305113
GGGTAGG37950.010.3062365
TTGAGGT284500.010.2943987
CGCGTAT19950.010.2693361
TTTGAGG315350.010.1437026
TTAAACG86050.010.1186682
GGGCCCT36000.010.0884566
AGGGCGG40600.09.9775939
Produced by FastQC (version 0.11.5)