FastQCFastQC Report
Tue 21 Jan 2020
5X.1.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename5X.1.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24417254
Sequences flagged as poor quality0
Sequence length20-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG73850.020.54223894
CGGGAAC110900.015.01181
GGGGGAT67750.014.5515031
CGTGCAT47350.011.44248294
TAGGGCG25200.011.0721518
GTTTTAT161350.010.9808831
CTTAAAC173200.010.8739771
TTGAGGT264300.010.5568757
GTTTTTT251900.010.1904231
GGGGATA92400.010.115922
CGCAAAC96900.010.0300661
TTAAACG97050.09.9666472
CTCGAAT144950.09.8492121
GGCGCCC63250.09.76437566-67
CGCGTAT16700.09.7461441
CGCGTTT49850.09.7017361
GTTTTTA160850.09.5405661
CTTTTAT163950.09.5303561
CTTTATT144050.09.4587921
TGAGGTT283100.09.4287328
Produced by FastQC (version 0.11.5)