FastQCFastQC Report
Tue 21 Jan 2020
5X.2.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename5X.2.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24291574
Sequences flagged as poor quality0
Sequence length20-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG64150.036.4728694
GGGGGAT63950.021.0524521
GGGGATA79450.016.1830562
TAGGGCG30250.015.0919768
CGGGAAC137200.014.2267371
CGTGCAT52850.012.71047294
GTAGGGC34700.012.6195487
CGCGTAT16850.012.4410781
CTTAAAC163050.011.5998031
GTTTTAT160400.010.77494051
GTTTTTT285150.010.6190431
CGGGGAC71400.010.4391981
TTGAGGT292750.010.422937
AGGGCGG46000.010.32979
GGGTAGG44450.010.2706925
GGGAACA180200.010.2631612
ATAAAGT137450.010.1337896
TTTGAGG330050.09.95072656
GGGATAA131300.09.7569333
GTTTGAT333350.09.740421
Produced by FastQC (version 0.11.5)