FastQCFastQC Report
Tue 21 Jan 2020
5X.2.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename5X.2.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24291574
Sequences flagged as poor quality0
Sequence length20-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG79250.028.40317594
GGGGGAT66850.015.2920071
CGGGAAC112650.015.0558371
TAGGGCG30900.014.586688
GTAGGGC35500.012.8274697
CGTGCAT50100.012.14921794
AGGGCGG41500.012.0925789
GGGTAGG40150.011.5733125
CGCGAAC30600.011.2370821
GGGGATA94700.010.7457662
GTTTTAT152250.010.5904741
CGGGGAC71850.010.5415391
CGCGTAT17200.010.5360881
CTTAAAC165600.010.4662641
GGGAACA151050.010.2746952
GTTTTTT245000.010.2227071
TTAAACG89800.010.1419852
TTGAGGT273900.09.9753597
GGGCCCT31450.09.75138958
TAAACGT84100.09.55857853
Produced by FastQC (version 0.11.5)