FastQCFastQC Report
Tue 21 Jan 2020
5X.3.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename5X.3.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24462881
Sequences flagged as poor quality0
Sequence length20-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG62700.037.0731994
GGGGGAT56950.016.1970141
CGGGAAC127550.013.4409771
CGTGCAT53550.011.03768594
GTTTTAT160000.010.9479131
TAAGTCG43350.010.88161194
GGGGATA78300.010.7691222
CGCGTAT18700.010.463361
CTTAAAC161100.010.2080471
CAAGTCG91500.010.18654694
TTGAGGT279500.010.0174267
GTTTTTT279300.010.0079191
CTTTTAT174100.09.7669191
TAGGGCG24900.09.7289858
GTTTGAT331100.09.6663171
AGTTTTG286900.09.6453823
TTTGAGG310150.09.5231576
CGGGAAT92050.09.3629231
GGAAAAT245850.09.247261
CTCGAAT152550.09.1921641
Produced by FastQC (version 0.11.5)