FastQCFastQC Report
Tue 21 Jan 2020
5X.3.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename5X.3.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24462881
Sequences flagged as poor quality0
Sequence length20-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG86250.026.65837394
CGGGAAC108600.015.1320221
GGGGGAT67100.012.4882631
TAGGGCG27250.011.6170138
GTTTTAT168700.011.3693121
CGCGTAT18800.010.89549351
CTTAAAC171050.010.8320861
TTGAGGT281550.010.7144917
TTTAATA106400.010.325772
TTAATAC61550.010.2107533
CAAGTCG96850.010.12470194
GGAAAAT241950.010.1207381
ATTTAAT89200.010.0204781
GTTTTTT266350.09.8927221
TTAAACG90050.09.8741952
TTTGAGG324650.09.7939386
CTTTTAT169100.09.7181411
TAAACGT86000.09.6354873
GTTTTTA160000.09.6307491
CTTAAAT103400.09.5448121
Produced by FastQC (version 0.11.5)