FastQCFastQC Report
Tue 21 Jan 2020
Ler.1.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLer.1.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24454018
Sequences flagged as poor quality0
Sequence length20-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG62550.035.5442694
GGGGGAT64350.015.2789311
TAGGGCG27900.011.5240198
CGGGAAC125700.011.2322421
TTAAACG82200.011.1674372
TTAATAC59200.011.0195893
GTTTTAT158250.010.9536371
GTTTTTT282050.010.8707341
GTTTGAT320150.010.5813251
CTTAAAC158000.010.5580811
TTGAGGT279650.010.4474867
GGGGATA84850.010.434242
TTTGAGG310900.010.08686
CGTGCAT49950.010.07995994
CTTAGAT110650.09.8963611
TTTAATA99500.09.8345682
CTTTTAT167850.09.7996931
GGAAAAT240650.09.6815131
GTTTTTA156050.09.6449021
CAGACGT48000.09.6106573
Produced by FastQC (version 0.11.5)